Biomedical waste management became a major issue because it poses potential health risks. Mortality due to infectious disease is the leading cause of deaths worldwide

There is no such thing as “away”. When we throw anything away it must go somewhere.

Annie Leonard

As you have read about biomedical waste in my previous blog. So this is my second blog on procedure, treatment, and disposal of biomedical waste management. We have to think of suitable alternatives to tackle this problem. Proper management of biomedical waste ensures the safety and hygiene of institutions and communities.

 Improper management can cause various viral or bacterial infections. This can lead to forming resistant organisms or pathogens. This further leads the organisms to our doorsteps. So, prime attention should be on the proper and safe disposal of these wastes. 

There’s a lot of confusion among different levels of management. It can be due to inappropriate awareness. It was mentioned in the Springer journal in 2002, an assessment run by W.H.O. in 22 developing countries. It found that 18% to 64% of healthcare facilities don’t use proper disposal methods.

Biomedical Waste (Management and Handling) July 1998 is the guideline issued by the Indian government. That is for safety management from storage to disposal of biomedical wastes. This rule has undergone revision and modification. Biomedical Waste Management in March 2016 is the latest amended edition. Under this rule, steps are defined for the proper management of biomedical wastes. I have mentioned these steps in detail below :

Procedure of Biomedical Waste Management 

The crucial steps for scientific and proper management of biomedical wastes are:

  •  Handling 
  • Segregation
  • Accumulation and storage
  • Treatment
  • Transportation
  • Disposal of waste

I have mentioned these steps in detail below :

Observation of Biomedical Waste 

Observation of waste

 Observation of waste

Waste should be observed carefully,from the point of generation to type of waste. Level of generation and disinfection determines the process of treatment of waste.

Segregation of waste 

Separation of different types of waste is essential. It reduces the risk of infection among workers and the public. In addition, it avoids physical injury. Color coding is not only for us. It represents the basic principle that all the waste is not the same so they will not end up in the same place. Different kinds of waste means their treatment process is also not the same. For the intention of segregation colour coded bins are used. Every bin has a distinct waste base on their treatment process and their quality. To know more about the Segregation of waste, visit our Consultation Website.

segregation of biomedical waste in different colour bins.
segregation of biomedical waste in different colour bins.

Red bins:- 

It is used to store contaminated wastes that can be recycled. These bags or bins should be non chlorinated since chlorinated bags can react with the waste. These are used for collection of following:

Research lab waste
Research lab waste
  • Catheters
  • Tubes
  • Cannulae
  • Syringes
  • Plastic IV bottles and sets
  • Rubber gloves
  • Specimen containers
  • Infected plastics
  • Lab wastes
  • Microbiology culture
  • Used discarded blood bags/ blood products
  • Vaccines, etc.

Yellow bins:- 

The yellow coloured bins are used to store many type of wastes like:

Biomedical waste container labeling
Biomedical waste container labeling
  1. Pathological waste 
  2. Solid (infectious) waste 
  3. Medical Chemical waste 
  4. Clinical Lab wastes 
  5. Pharmaceutical waste

These waste are stored in differently marked bins. Biohazard, radioactive, and cytotoxic marks are some examples of different marks. Some examples that comes under this category are mentioned below :

Antibiotic wastes
Antibiotic wastes
  • Human anatomical wastes
  • Expired medicine waste like cytotoxic drugs along with vials
  •  Antibiotics
  • Liquid wastes 
  •  Disinfectant wastes 
  •  Silver X- ray liquid 
  • Formalin solutions 
  •  Infected secretions 
  •  Aspirated body fluids 
  •  Floor washing liquids 
  •  Liquids from laboratories 
  • Soiled cotton swabs, bandages, dressing pads, contaminated gauze piece
  •  Discarded linen beddings and mattress
  • Biotechnological or microbiological lab wastes, etc.

White Bins :- 

 These are white or translucent puncture proof bags. These bags are used to store sharps like :

  • Needles 
  • Syringes with fixed needles
  • Needle from needle cutter or burner
  • Scalpels
  • Blades
  • Razors
  • Nails
  • Contaminated sharp metal objects, etc.

Blue bin:- 

Blue bins are used to store glassware whether medicinal or general. Some example are like:

Broken and infected glassware
Broken and infected glassware
  • Infected broken glass/ bottle
  • Broken or unbroken glass
  • Vials
  • Ampules
  • Glass IV bottles
  • Glass injections
  • Metallic body implants
  • Slides
  • Glassware used in wards 
  • Glass injections or syringes, etc.

Accumulation and Storage 

Waste bag
Waste bag

Storing waste is for collection purposes. Accumulation stands for holding of waste for a short time near the point of waste generation. Storage stands for long term storing of wastes near the waste treatment or disposal site.

Transportation of waste

transporting of waste in closed vehicle
transporting of waste in closed vehicle

 Wastes are mobilized from the generation point to the treatment disposal site via a designated vehicle. To avoid public exposure, smell, and microbes, containers must always be covered. All the vehicles used by CBWTF operators should not be sub-letters. Operators cannot use a contract vehicle. CBWTF operator’svehicles should only be used for waste collection purposes. CBWTF operator’s vehicles should be registered under Motor Vehicle Act with the R.T.O/ TransportDepartment. Such vehicle numbers should be registered with respective SPCB/PCC. Closed vehicles should be used. 

Treatment of waste

Treatment signifies the modification of waste before sending it to its final resting place. Treatment should be at the source of generation. Treatment is making waste safe through some process to make it no longer a source of the pathogen. Some of the processes are bleaching, shredding, and chemical disinfecting. Indian govt. Has issued a set of guidelines for waste treatment. After treatment, the residual can be transported to their disposal site. Some common treatments of waste are:

treatment of wastes
treatment of wastes
  • Needles and syringes nozzles should shred into a needle destroyer or syringe cutter. 
  • Glassware should be clean, disinfected and sterilized. 
  • Scalpel, razor, blades, broken glass should be stored in a puncture proof container with bleach. Then transferred into labelled plastic or cardboard box . These boxes should be sealed to prevent spillage, and further send it to incubators. 
  • Culture plates are disposed of into appropriate bags. And plates should be autoclave and then sterilized. Sterilized plates can be reused. 
  • Gloves must be shredded, mutilated, cut and destroyed; before disposal. 

Disposal of Biomedical Wastes

Dumping of waste into landfill
Dumping of waste into landfill

Disposal methods differ in availability, efficiency, cost effectiveness and impacts on the environment. Biomedical waste should be pathogen free before disposal. This should ensure utmost public health and hygiene. 

Less developed countries are advised to use solar heating systems for disinfecting infectious wastes. This method results in considerable reduction in the number of viable bacteria but not completely. That still poses a threat. Disposal without treatment is non- advisable for anatomical, sharps and waste from labs. Various disposal methods are: 

1). Incinerators: 


This is a process of burning waste at a high temperature. The temperature in incinerators ranges from 1,800 degrees F to 2,000 degrees F (982 degrees C to 1093 degrees C). Incinerators have the advantage of quick, easy disposal methods.

 But their major concern is due to emissions. Many states and localities have postponed the use of incinerators for the time being until further information on safety options is available. 

2). Autoclaving: 

Autoclaving is comparable to steam sterilization. It is the most commonly used alternative to incineration. It is cost-effective as well as has no reported health impacts. Waste is sterilized or disinfected before being dumped in landfills. Waste bags are placed in chambers. Then steam is started for a specific time at a definite temperature and pressure. 

This method has assurity in the destruction of microorganisms. Approx 90% of biomedical wastes are suitable for autoclaving, particularly microbiological wastes. Autoclaving is not suitable for cytotoxic, pathological, radiotoxic, or other toxic chemical wastes


3).Gas sterilization:

Gas chambers
Gas chambers

In this process, medical waste is placed into an empty air-tight chamber. Treated with a sterilizing agent (such as ethylene oxide or formaldehyde). Gas emitted from these chemicals comes in contact with waste. In turn it kills harmful, infectious agents. 

EPA does not recommend ethylene oxide for infectious waste because of its toxicity. 

4). Chemical disinfection:

This method is preferred for liquid waste; it can also be used for solid waste. In this method chemical agents are use for disinfection of wastes like chlorine.

chemical treatment of waste
chemical treatment of waste

There are various factors considered about the effectiveness including :

  • Type of disinfectant used
  • Degree of contamination
  • Type and morphology of microorganisms 
  • Chemical’s concentration, quantity, contact time and requirements for mixing

Alternatives are grinding of wastes before introduction with a liquid chemical disinfectant. Grinding confirms sufficient subjection of chemical disinfectant to all parts of waste. That aids in the easy disposal of residues. The resulting liquid is drained into sewers, and solids are dumped into landfills.

5). Microwave: 

Microwave technology can also disinfect waste. Wastes are first placed into a shredder. These wastes are mixed with water and internally heated, which neutralizes present biologicals. Microwave units are controlled through computers. That ensures the least parameters of infection and proper equipment function. 90% of medical wastes can be autoclaved. 

The amount of residue produced is low because of shredding. Requires less energy than incinerators. 

6). Irradiation: 

This method involves waste sterilization by exposing waste to cobalt sources. Cobalt emits gamma rays that kill all the microbes in waste. 

Commercial companies don’t use this technology due to high cobalt cost and operating costs. Some queries are on the efficiency of proper disinfecting and radiating materials with rays. It is not recommended for pathological wastes.


 Thermal Inactivation: 

This process involves heat waste at high temperatures resulting in the killing of microbes. These methods are advised for treating large amounts of liquid waste. A chamber is preheated to an intense specific temperature at a specific amount of time and then released. 

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Safe and effective management of biomedical waste is not only a lawful obligation but also a civic duty. Appropriate gathering and segregation of biomedical wastes are vital. The quantity of waste generated is also critical. Lesser quantity of biomedical waste signifies less burden on disposal work. That would be more money and labor-saving and a more well-structured waste disposal system.

We should step up efforts to reduce the waste generation in day-to-day work in clinics and hospitals.

In my next blog, I will be covering biomedical waste management problems and their solutions. If you want a legal procedure in the handling of biomedical waste, you can see our blog Disposing of Biomedical Waste: Authorities’ Guidelines


The benefits of biomedical progress are obvious, clear and powerful. The hazards are much less appreciated. 

Leon Kass

In case I missed anything please feel free to mention in the comments. I would love to hear your inputs on the subjects. If you want to know more about the most concerning issues, stay updated on our website. Subscribe with us.

andleep zahra

She is a biotechnologist and received her master degree in biotechnology from V.B.S. Purvanchal University. Being a biotechnologist she loves to do research and write articles on the same. She’s best known for writing articles and blogs on environmental issues. Through her writings she likes to provide more information on environmental conservation and provide knowledge on how to address the issue, mainly focused on waste management. She looks for ways to get involved and also attended several conferences, workshops and webinars. “Increasing waste around the globe that is affecting our ecosystem and reducing our biodiversity is the most critical issue we humans often overlook and it will be the greatest challenge our younger generation will ever face,” she says.

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